Human PAK6 kinase domain (Q9NQU5, 385 a.a. - 680 a.a.) partial recombinant protein expressed in Escherichia coli.
GPHMVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTST The first 4 residues GPHM are from Turbo3C Protease cleavage site. The underlined S is phosphorylated S560.
Theoretical MW (kDa):
Escherichia coli expression system
Specific activity: 2,086 pmoles/min/ug
Quality Control Testing:
Loading 7 ug protein in SDS-PAGE
In 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Result of activity analysis
Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen): 1. Different concentrations of PAK6 were incubated in a buffer containing 50 mM HEPES pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 14, Invitrogen) at RT for 1 hour. 2. Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at RT. 3. Fluorescence was then detected using λexc=460±40 nm and λem=528±20 nm filters.
This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
p21(CDKN1A)-activated kinase 6,p21-activated kinase 6,p21-activated protein kinase 6