IGF1 (Human) Recombinant Protein
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Specification
Product Description
Human IGF1 (P05019) recombinant protein expressed in Escherichia coli.
Sequence
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA
Host
Escherichia coli
Theoretical MW (kDa)
7.7
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent proliferation of FDC-P1 cells. The expected ED50 for this effect is 0.65-0.98 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — IGF1
Entrez GeneID
3479Protein Accession#
P05019Gene Name
IGF1
Gene Alias
IGFI
Gene Description
insulin-like growth factor 1 (somatomedin C)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene
Other Designations
insulin-like growth factor 1|somatomedin C
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