LGALS1 (Human) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
Human THP-1 cells were allowed to migrate to human LGALS1 at (0, 0.1, 1, 10, 100 and 1000 ng/mL). After 1 hour, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above.
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Specification
Product Description
Human LGALS1 (P09382) recombinant protein expressed in Escherichia coli.
Sequence
MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD
Host
Escherichia coli
Theoretical MW (kDa)
14.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the ability to induce chemotaxis of human THP-1 cells and is detectable starting at 100 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
Lyophilized from 10 mM Na2PO4, pH 7.5
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — LGALS1
Entrez GeneID
3956Protein Accession#
P09382Gene Name
LGALS1
Gene Alias
DKFZp686E23103, GAL1, GBP
Gene Description
lectin, galactoside-binding, soluble, 1
Omim ID
150570Gene Ontology
HyperlinkGene Summary
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq
Other Designations
OTTHUMP00000028982|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|galaptin|galectin 1|galectin-1|lactose-binding lectin 1
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Interactome
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Disease
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