Human SRC (P12931) full-length recombinant protein expressed in Sf9 cells.
Theoretical MW (kDa):
85
Form:
Liquid
Preparation Method:
Insect cell (Sf9) expression system
Purity:
≥ 90% by SDS-PAGE
Activity:
116 nmol phosphate incorporated into Src substrate peptide per minute per mg proteins at 30°C for 15 minutes using a final concentration of 50 uM ATP (0.83uCi/assay in 25uL reaction volume).
Quality Control Testing:
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer:
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM Dithiothreitol, 0.1 mM EGTA, 0.1 mM EDTA, 25% glycerol, 0.1 mM PMSF)
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq