NTF3 (Human) Recombinant Protein
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Specification
Product Description
Human NTF3 (P20783, 139 a.a. - 257 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT
Host
Escherichia coli
Theoretical MW (kDa)
27 (non-reducing con
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 10-50 ng/mL.
Storage Buffer
Lyophilized from PBS, pH 7.5
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — NTF3
Entrez GeneID
4908Protein Accession#
P20783Gene Name
NTF3
Gene Alias
HDNF, MGC129711, NGF-2, NGF2, NT3
Gene Description
neurotrophin 3
Omim ID
162660Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq
Other Designations
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