TNF (Human) Recombinant Protein
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Specifications
Product Description
Human TNF (NP_000585, 77 a.a. - 233 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Host
Escherichia coli
Theoretical MW (kDa)
17.5
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is < 0.16 ng/mL.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — TNF
Entrez GeneID
7124Protein Accession#
NP_000585Gene Name
TNF
Gene Alias
DIF, TNF-alpha, TNFA, TNFSF2
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations
APC1 protein|OTTHUMP00000029281|OTTHUMP00000037669|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
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Interactomes
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Pathways
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Diseases
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Publication Reference
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IFN-γ and IL-18 in conditioned media of parasite-infected host and IL-21-silenced colorectal cancer cells.
Peng Ge, Ching Yi Ong, Abdalla Eshtiyag Abdalkareem, Boon Yin Khoo, Bo Yuan.
Experimental and Therapeutic Medicine 2021 Feb; 21(2):103.
Application:Quant, Human, HCT-116 cells, Human foreskin fibroblasts.
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IFN-γ and IL-18 in conditioned media of parasite-infected host and IL-21-silenced colorectal cancer cells.
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