NUP98 monoclonal antibody, clone 2H10
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of NUP98 protein with NUP98 monoclonal antibody, clone 2H10 (Cat # MAB6758). Sample is HeLa nuclear membrane fraction. The IgG solution was 2,000-fold diluted before use.
Immunofluorescence
Immunofluorescent staining of rat neuron with NUP98 monoclonal antibody, clone 2H10 (Cat # MAB6758). The dots are NUP98.
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Specification
Product Description
Rat monoclonal antibody raised against partial recombinant NUP98.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 1-466 of human NUP98.
Host
Rat
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG2c, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (50% glycerol, azide free).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Detection of NUP98 protein with NUP98 monoclonal antibody, clone 2H10 (Cat # MAB6758). Sample is HeLa nuclear membrane fraction. The IgG solution was 2,000-fold diluted before use.Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of rat neuron with NUP98 monoclonal antibody, clone 2H10 (Cat # MAB6758). The dots are NUP98.Enzyme-linked Immunoabsorbent Assay
Dot Blot
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Gene Info — NUP98
Entrez GeneID
4928Protein Accession#
Q9HDC8Gene Name
NUP98
Gene Alias
ADIR2, NUP196, NUP96
Gene Description
nucleoporin 98kDa
Omim ID
601021Gene Ontology
HyperlinkGene Summary
Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kD nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kD precursor protein. This cleavage results in the 98 kD nucleoporin as well as a 96 kD nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kD nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromsome translocatons in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq
Other Designations
GLFG-repeat containing nucleoporin|Nup98-Nup96|OTTHUMP00000013819|OTTHUMP00000013967|nucleoporin 98kD
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Interactome
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Disease
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Publication Reference
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Differential functions of calpain 1 during epithelial cell death and adipocyte differentiation in mammary gland involution.
Arnandis T, Ferrer-Vicens I, Torres L, Garcia C, Garcia-Trevijano ER, Zaragoza R, Vina JR.
The Biochemical Journal 2014 Apr; 459(2):355.
Application：WB-Ti, Mouse, Mammary gland.
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NUP98 dysregulation in myeloid leukemogenesis.
Moore MA, Chung KY, Plasilova M, Schuringa JJ, Shieh JH, Zhou P, Morrone G.
Annals of the New York Academy of Sciences 2007 Jun; 1106:114.
Application：IF, Human, Human leukemic cells.
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Specific monoclonal antibody against the nuclear pore complex protein, nup98.
Fukuhara T, Ozaki T, Shikata K, Katahira J, Yoneda Y, Ogino K, Tachibana T.
Hybridoma (2005) 2005 Oct; 24(5):244.
Application：ELISA, WB, Human, HeLa cells.
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Nup98 is a mobile nucleoporin with transcription-dependent dynamics.
Griffis ER, Altan N, Lippincott-Schwartz J, Powers MA.
Molecular Biology of the Cell 2002 Apr; 13(4):1282.
Application：EM, IF, WB-Tr, Human, XL177, HeLa cells.
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Differential functions of calpain 1 during epithelial cell death and adipocyte differentiation in mammary gland involution.
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