TOP2A monoclonal antibody, clone 3F6
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining for paraffin-embedded human bladder tumor section using TOP2A monoclonal antibody, clone 3F6 (Cat # MAB9689). Note intense nuclear staining of malignant cells and occasional mitotic figures.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant TOP2A.
Immunogen
Recombinant protein corresponding to the C-terminal region of TOP2A.
Host
Mouse
Reactivity
Human
Form
Lyophilized
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:20-1:40)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from tissue culture supernatant (0.09% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with steril distilled water, Store at 4°C for at least two months.
For long term storage store at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining for paraffin-embedded human bladder tumor section using TOP2A monoclonal antibody, clone 3F6 (Cat # MAB9689). Note intense nuclear staining of malignant cells and occasional mitotic figures.Immunohistochemistry (Frozen sections)
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Gene Info — TOP2A
Entrez GeneID
7153Gene Name
TOP2A
Gene Alias
TOP2, TP2A
Gene Description
topoisomerase (DNA) II alpha 170kDa
Omim ID
126430Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq
Other Designations
DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha (170kD)
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Interactome
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Disease
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Publication Reference
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nm23, TOP2A and VEGF expression: Potential prognostic biologic factors in peripheral T-cell lymphoma, not otherwise specified.
Huang W, Cao Z, Zeng L, Guo L, Liu X, Lv N, Feng X.
Oncology Letters 2019 Oct; 18(4):3803.
Application：IHC-P, Human, T‑cell lymphoma.
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nm23, TOP2A and VEGF expression: Potential prognostic biologic factors in peripheral T-cell lymphoma, not otherwise specified.
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