Mouse polyclonal antibody raised against synthetic peptide of ERBB3.
A synthetic peptide corresponding to cytoplasmic region of human ERBB3.
Immunohistochemistry (1:20-1:40) The optimal working dilution should be determined by the end user.
Lyophilized from tissue culture supernatant (15 mM sodium azide)
Store at 4°C on dry atmosphere. After reconstitution with steril distilled water, Store at 4°C for at least two months. For long term storage store at -20°C or below. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining for paraffin-embedded human rectal adenocarcinoma section using ERBB3 monoclonal antibody, clone RTJ1 (Cat # MAB9533). Note cytoplasmic staining of characteristic in these tumor. In other tumor types, membrane staining is obseved.
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq