CLEC4E monoclonal antibody, clone AT16E3
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: MINCLE transfected 293T cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescence of human HeLa cells stained with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
Flow Cytometry
Flow cytometry analysis of LNCap cell line, staining at 2-5 ug for 1x106cells. (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CLEC4E.
Immunogen
Recombinant protein corresponding to amino acids 41-219 of human CLEC4E.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: MINCLE transfected 293T cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunocytochemistry
Immunofluorescence
Immunofluorescence of human HeLa cells stained with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of LNCap cell line, staining at 2-5 ug for 1x106cells. (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — CLEC4E
Entrez GeneID
26253Protein Accession#
NP_055173Gene Name
CLEC4E
Gene Alias
CLECSF9, MINCLE
Gene Description
C-type lectin domain family 4, member E
Omim ID
609962Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq
Other Designations
C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9|macrophage-inducible C-type lectin
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Interactome
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Publication Reference
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Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle.
Ishikawa E, Ishikawa T, Morita YS, Toyonaga K, Yamada H, Takeuchi O, Kinoshita T, Akira S, Yoshikai Y, Yamasaki S.
The Journal of Experimental Medicine 2009 Dec; 206(13):2879.
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Mincle is a long sought receptor for mycobacterial cord factor.
Matsunaga I, Moody DB.
The Journal of Experimental Medicine 2009 Dec; 206(13):2865.
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The Dectin-2 family of C-type lectins in immunity and homeostasis.
Graham LM, Brown GD.
Cytokine 2009 Aug; 48(1-2):148.
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Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle.
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