Mouse monoclonal antibody raised against partial recombinant EPHA1.
Immunogen:
Recombinant protein corresponding to truncated human EPHA1.
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
Western Blot (1:200-1:1000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000) Immunohistochemistry (Frozen sections) (1:200-1:1000) ELISA (1:10000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embbed human ovary carcinoma (A) and breast carcinoma (B) tissue, showing cytoplasmic localization using EPHA1 monoclonal antibody, clone 5D2D1 (Cat # MAB8303).
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq