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Last updated: 2014/10/20
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EPHA1 monoclonal antibody, clone 5D2D1

  • Catalog # : MAB8303
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant EPHA1.
  • Immunogen:
  • Recombinant protein corresponding to truncated human EPHA1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (1:200-1:1000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
    Immunohistochemistry (Frozen sections) (1:200-1:1000)
    ELISA (1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of A-549 (Lane 1) and HeLa (Lane 2) cell extracts with EPHA1 monoclonal antibody, clone 5D2D1 (Cat # MAB8303).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embbed human ovary carcinoma (A) and breast carcinoma (B) tissue, showing cytoplasmic localization using EPHA1 monoclonal antibody, clone 5D2D1 (Cat # MAB8303).
  • Immunohistochemistry (Frozen sections)
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2041
  • Gene Name:
  • EPHA1
  • Gene Alias:
  • EPH,EPHT,EPHT1,MGC163163
  • Gene Description:
  • EPH receptor A1
  • Gene Summary:
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq
  • Other Designations:
  • eph tyrosine kinase 1,ephrin receptor EphA1,ephrin type-A receptor 1,erythropoietin-producing hepatoma amplified sequence,oncogene EPH,soluble EPHA1 variant 1,soluble EPHA1 variant 2,tyrosine-protein kinase receptor EPH
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