ABCA1 monoclonal antibody, clone AB1.G6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblasts from different patients (Lane 1, 2, 3, 4) with ABCA1 monoclonal antibody, clone AB1.G6 (Cat # MAB8291). Stimultaneous blotting with anti-actin antibody used for protein loading control.
Immunohistochemistry
Immunoreactivity of ABCA1 in adipose (A) and pancreas (B) tissue for ABCA1 protein.
A. Strong immunopositivity of excrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly postive (data not shown).
B. The negative control for Panel A.
Original magnification 300 fold; hematoxylin counterstain.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of ABCA1.
Immunogen
A synthetic peptide corresponding to amino acids 1800-2260 of ABCA1.
Host
Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblasts from different patients (Lane 1, 2, 3, 4) with ABCA1 monoclonal antibody, clone AB1.G6 (Cat # MAB8291). Stimultaneous blotting with anti-actin antibody used for protein loading control.Immunohistochemistry
Immunoreactivity of ABCA1 in adipose (A) and pancreas (B) tissue for ABCA1 protein.
A. Strong immunopositivity of excrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly postive (data not shown).
B. The negative control for Panel A.
Original magnification 300 fold; hematoxylin counterstain.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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MiR-26 controls LXR-dependent cholesterol efflux by targeting ABCA1 and ARL7.
Sun D, Zhang J, Xie J, Wei W, Chen M, Zhao X.
FEBS Letters 2012 May; 586(10):1472.
Application：WB-Tr, Human, Mouse, RAW-LXRa, THP-1 cells.
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MiR-26 controls LXR-dependent cholesterol efflux by targeting ABCA1 and ARL7.
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