Mitochondria monoclonal antibody, clone 113-1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of HeLa lysate showing specific immunolabeling of the ~ 60k mitochondrial protein.
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Specification
Product Description
Mouse monoclonal antibody raised against native Mitochondria.
Immunogen
Native purified from human cell homogenate.
Host
Mouse
Theoretical MW (kDa)
60
Reactivity
Human
Specificity
This antibody has been directly tested for reactivity in human tissue. It does not react with mouse or rat mitochondria. specific to human mitochondria in all cell types. This antibody works on acetone fixed and 2% paraformaldehyde fixed cells. This antibody also works for immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) cryostat sections.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:800)
Immunohistochemistry (1:800)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of HeLa lysate showing specific immunolabeling of the ~ 60k mitochondrial protein.Immunohistochemistry
Immunofluorescence
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Publication Reference
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Mitochondria: more than just a powerhouse.
McBride HM, Neuspiel M, Wasiak S.
Current Biology 2006 Jul; 16(14):R551.
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Apoptotic pathways: the roads to ruin.
Green DR.
Cell 1998 Sep; 94(6):695.
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Mitochondria: more than just a powerhouse.
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