5 X 105 cultured Raji human tumor cells were incubated 45 minutes on ice with 80 uL of CD40 monoclonal antibody, clone EA-5 (Cat # MAB6912) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
B cell surface antigen CD40,B cell-associated molecule,CD40 antigen,CD40 antigen (TNF receptor superfamily member 5),CD40 type II isoform,CD40L receptor,OTTHUMP00000031699,OTTHUMP00000031700,nerve growth factor receptor-related B-lymphocyte activation mol