HAX1 monoclonal antibody, clone AT3C5
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More Files
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Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with HAX1 monoclonal antibody, clone AT3C5 (Cat # MAB6510) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with HAX1 monoclonal antibody, clone AT3C5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised aganist full length recombinant HAX1.
Immunogen
Recombinant protein corresponding to full length human HAX1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with HAX1 monoclonal antibody, clone AT3C5 (Cat # MAB6510) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with HAX1 monoclonal antibody, clone AT3C5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HAX1
Entrez GeneID
10456Protein Accession#
NP_006109Gene Name
HAX1
Gene Alias
FLJ17042, FLJ18492, FLJ93803, HCLSBP1, HS1BP1, SCN3
Gene Description
HCLS1 associated protein X-1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HCLS1 (and PKD2) associated protein|HS1 binding protein|OTTHUMP00000034190
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Interactome
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Disease
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Publication Reference
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HAX1 deficiency causes autosomal recessive severe congenital neutropenia (Kostmann disease).
Klein C, Grudzien M, Appaswamy G, Germeshausen M, Sandrock I, Schaffer AA, Rathinam C, Boztug K, Schwinzer B, Rezaei N, Bohn G, Melin M, Carlsson G, Fadeel B, Dahl N, Palmblad J, Henter JI, Zeidler C, Grimbacher B, Welte K.
Nature Genetics 2007 Jan; 39(1):86.
Application：WB, Human, EBV B cell lines SCN-I, SCN-II, SCN-III.
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Evidence that HAX-1 is an interleukin-1 alpha N-terminal binding protein.
Yin H, Morioka H, Towle CA, Vidal M, Watanabe T, Weissbach L.
Cytokine 2001 Aug; 15(3):122.
Application：WB-Re, WB-Tr, Human, HEK 293 cells, Recombinant protein.
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HAX1 deficiency causes autosomal recessive severe congenital neutropenia (Kostmann disease).
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