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Last updated: 2014/10/27
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EGFR (phospho Y1173) monoclonal antibody, clone EM-13

  • Catalog # : MAB6475
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of EGFR.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y1173 of human EGFR.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts with human EGFR (ErbB1/HER1) phosphorylated on tyrosine 1173.
  • Form:
  • Liquid
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry
    Immunoprecipitation
    Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (15 mM sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
    Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blotting analysis of EGFR (phospho-Tyr1173) by EGFR (phospho Y1173) monoclonal antibody, clone EM-13 (Cat # MAB6475) in EGF-treated A-431 (Lane 1), CALU-3 (Lane 2), MCF-7 (Lane 3), Jurkat (Lane 4) and Ramos (Lane 5) cell lines (reduced conditions).
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of EGFR from EGF-treated A-431 cells by EGFR (phospho Y992) monoclonal antibody, clone EM-12 (Cat # MAB6476) (Lane 1), EGFR (phospho Y1173) monoclonal antibody, clone EM-13 (Cat # MAB6475) (Lane 2). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 1956
  • Gene Name:
  • EGFR
  • Gene Alias:
  • ERBB,ERBB1,HER1,PIG61,mENA
  • Gene Description:
  • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • Gene Summary:
  • The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
  • Other Designations:
  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor
  • Interactome
  • Interactome
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