CTNNB1 monoclonal antibody, clone EM-22
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More Files
- More Functions
Western Blot (Cell lysate)
Western blotting analysis of beta-catenin in murine 3T3 (Lane 1), C57 (Lane 2) and KW1 (Lane 3) cell lines using CTNNB1 monoclonal antibody, clone EM-22 (Cat # MAB6470).
Immunofluorescence
Immunofluorescence staining of beta-catenin in (human colon adenocarcinoma cell line) HT29 using CTNNB1 monoclonal antibody, clone EM-22 (Cat # MAB6470) (green). Cell nuclei visualized by DAPI (blue).
Flow Cytometry
Flow cytometric analysis (intracellular) of beta-catenin in human MCF-7 cell line by CTNNB1 monoclonal antibody, clone EM-22-Alexa Fluor® 488 (red) compared to isotype control (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant CTNNB1.
Immunogen
Recombinant protein corresponding to human CTNNB1.
Host
Mouse
Reactivity
Hamster, Human, Mouse
Specificity
This antibody reacts with C-terminal part of beta-catenin, an 88 KDa multi functional protein involved both in cell adhesion and in activation of transcription.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blotting analysis of beta-catenin in murine 3T3 (Lane 1), C57 (Lane 2) and KW1 (Lane 3) cell lines using CTNNB1 monoclonal antibody, clone EM-22 (Cat # MAB6470).Immunofluorescence
Immunofluorescence staining of beta-catenin in (human colon adenocarcinoma cell line) HT29 using CTNNB1 monoclonal antibody, clone EM-22 (Cat # MAB6470) (green). Cell nuclei visualized by DAPI (blue).Immunoprecipitation
Flow Cytometry
Flow cytometric analysis (intracellular) of beta-catenin in human MCF-7 cell line by CTNNB1 monoclonal antibody, clone EM-22-Alexa Fluor® 488 (red) compared to isotype control (blue). -
Gene Info — CTNNB1
Entrez GeneID
1499Gene Name
CTNNB1
Gene Alias
CTNNB, DKFZp686D02253, FLJ25606, FLJ37923
Gene Description
catenin (cadherin-associated protein), beta 1, 88kDa
Gene Ontology
HyperlinkGene Summary
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM
Other Designations
OTTHUMP00000165222|OTTHUMP00000165223|catenin (cadherin-associated protein), beta 1 (88kD)|catenin beta-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches.
Nowak M, Madej JA, Dziegiel P.
Folia Histochemica et Cytobiologica 2007 Oct; 45(3):233.
Application：IHC-P, Human, Mammary tumour.
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Epstein-Barr virus, beta-catenin, and E-cadherin in gastric carcinomas.
Jung IM, Chung JK, Kim YA, Kim JE, Heo SC, Ahn YJ, Hwang KT, Kim BG, Lee KL, Kim CW, Kim WH, Chang MS.
Journal of Korean Medical Science 2007 Oct; 22(5):855.
Application：IHC-P, Human, Gastric carcinomas.
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HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells.
Valenta T, Lukas J, Korinek V.
Nucleic Acids Research 2003 May; 31(9):2369.
Application：Func, Human, HEK 293 cells.
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Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches.
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