Cd19 monoclonal antibody, clone 6D5 (SPRD)
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More Files
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Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd19 monoclonal antibody, clone 6D5 (FITC) (Cat # MAB5687) and Cd3e monoclonal antibody, clone C363.29B (PE) (Cat # MAB6102), gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Cd19.
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells.
Host
Rat
Reactivity
Mouse
Specificity
CD19 B-cell differentiation antigen, Mr 95 KDa.
Form
Liquid
Conjugation
SPRD
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd19 monoclonal antibody, clone 6D5 (FITC) (Cat # MAB5687) and Cd3e monoclonal antibody, clone C363.29B (PE) (Cat # MAB6102), gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd19
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Publication Reference
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The signaling activity of murine CD19 is regulated during cell development.
Krop I, Shaffer AL, Fearon DT, Schlissel MS.
Journal of Immunology 1996 Jul; 157(1):48.
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Self-renewal of B-1 lymphocytes is dependent on CD19.
Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT.
European Journal of Immunology 1996 Jan; 26(1):238.
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The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes.
D T Fearon.
Current Opinion in Immunology 1993 Jun; 5(3):341.
Application：Flow Cyt, Human, Human B lymphocytes.
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The signaling activity of murine CD19 is regulated during cell development.
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