CD14 monoclonal antibody, clone biG 13
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Specification
Product Description
Mouse monoclonal antibody raised against CD14.
Immunogen
Monocytes of different species and immunoaffinity purified soluble human CD14.
Host
Mouse
Reactivity
Bovine, Dog, Horse, Human, Pig
Specificity
biG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.
Characteristic: 20 ug/mL inhibits binding of 0.5 ug/mL LPS to CD14, binding epitope amino acid 9-13, 39-44. Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000. Inhibts LPS induced IL1beta and TNF-secretion.Form
Lyophilized
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA
Flow Cytometry
Western Blot
CD14 inhibition studies
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS.
Storage Instruction
Store at -20°C on dry atmosphere. For long term storage, store desiccated at -80°C.
After reconstitution with 100 uL water to get concentration of 1 mg/mL in PBS, reconstituted protein should be used immediately, or store in undiluted working aliquotes at -20°C. For long term storage, it is recommended to add carrier protein (0.1% BSA).
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — CD14
Entrez GeneID
929Gene Name
CD14
Gene Alias
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Gene Description
CD14 molecule
Omim ID
158120Gene Ontology
HyperlinkGene Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
Other Designations
CD14 antigen|monocyte differentiation antigen CD14
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Interactome
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Pathway
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Disease
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Publication Reference
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Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli.
Stelter F, Bernheiden M, Menzel R, Jack RS, Witt S, Fan X, Pfister M, Schutt C.
European Journal of Biochemistry 1997 Jan; 243(1-2):100.
Application:Func, Flow Cyt, Human, Mouse, CHO cells, Human monocytes.
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The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms.
Stelter F, Pfister M, Bernheiden M, Jack RS, Bufler P, Engelmann H, Schutt C.
European Journal of Biochemistry 1996 Mar; 236(2):457.
Application:Blocking, ELISA, Flow Cyt, Hamster, CHO cells.
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Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli.
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