KRT19 monoclonal antibody, clone SB39g
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More Files
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Immunofluorescence
Cell cytospin of the HCC38 breast carcinoma cell line was incubated with KRT19 monoclonal antibody, clone SB39g (Cat # MAB5631). After washing, a 10 ug/mL dilution of goat anti-mouse Ig-FITC was used for immunofluorescence staining. Positive cells were viewed by fluorescence microscopy.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KRT19.
Immunogen
Recombinant protein corresponding to C-terminus of human KRT19.
Host
Mouse
Reactivity
Human
Specificity
Cytokeratin 19.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Immunohistochemistry (10 ug/mL)
Western Blot (1:2000-1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunofluorescence
Cell cytospin of the HCC38 breast carcinoma cell line was incubated with KRT19 monoclonal antibody, clone SB39g (Cat # MAB5631). After washing, a 10 ug/mL dilution of goat anti-mouse Ig-FITC was used for immunofluorescence staining. Positive cells were viewed by fluorescence microscopy.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KRT19
Entrez GeneID
3880Gene Name
KRT19
Gene Alias
CK19, K19, K1CS, MGC15366
Gene Description
keratin 19
Omim ID
148020Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
40-kDa keratin intermediate filament|cytokeratin 19|keratin, type I cytoskeletal 19|keratin, type I, 40-kd
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Interactome
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Disease
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Publication Reference
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Expression of cytokeratin 19 in human hepatocellular carcinoma cell lines.
Wu F, Nishioka M, Fujita J, Murota M, Ohtsuki Y, Ishida T, Kuriyama S.
International Journal of Oncology 2002 Jan; 20(1):31.
Application：IHC, WB, Human, HepG2, Huh-7, HuCCT1, PLC/PRF/5, RBE cells.
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Expression of simple epithelial type cytokeratins in stratified epithelia as detected by immunolocalization and hybridization in situ.
Bosch FX, Leube RE, Achtstatter T, Moll R, Franke WW.
The Journal of Cell Biology 1988 May; 106(5):1635.
Application：IF, IHC-Fr, Human, Human esophagus, Humanv agina.
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Protein complexes of intermediate-sized filaments: melting of cytokeratin complexes in urea reveals different polypeptide separation characteristics.
Franke WW, Schiller DL, Hatzfeld M, Winter S.
PNAS 1983 Dec; 80(23):7113.
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Expression of cytokeratin 19 in human hepatocellular carcinoma cell lines.
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