This antibody is specific to the 46KDa polypeptide (cytokeratin 20). It represents an excellent marker for certain carcinomas such as adenocarcinoma of the colon, transitional cell carcinoma of the bladder and Merkel cell skin tumours. This antibody can be used in the detection of intestinal and gastric foveolar epithelium, urothelial cells and Merkel cells of the epidermis as well as tumours of their origin (e.g. primary and metastatic colorectal cancer). Adenocarcinomas of the breast and lung also neuroendocrine tumours of the lung are essentially negative.
Protein A purification
Recommend dilution at 1:20-1:50 The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide, 0.5% BSA)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq