Mouse monoclonal antibody raised against native CD14.
Immunogen:
Native purified human CD14.
Host:
Mouse
Theoretical MW (kDa):
53-55
Reactivity:
Human, Primates
Specificity:
This antibody reacts with CD14, a 53-55 KDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. This antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
Form:
Liquid
Conjugation:
PE
Isotype:
IgG1
Recommend Usage:
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.2% BSA, 15 mM sodium azide)
Storage Instruction:
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq