Mouse monoclonal antibody raised against native PTPRC.
Native purified PTPRC from human thymocytes and T lymphocytes.
This antibody reacts with the LCA complex expressed on all haemopoietic cells but not platelets (190, 205, 200 KDa). The CD45 molecule is also known as the Leukocyte Common Antigen (LCA) or T200 antigen, and is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells, e. g. granulocytes, monocytes, macrophages and lymphocytes, except mature erythroid cells. Detection of the different isoforms can distinguish, for example, between naive T cells and memory T cells, which is of interest in patients with immunodeficiency and autoimmune diseases. Variations in CD45RB expression can discriminate between Th1 and Th2 cells, i. e. CD45RB-bright and CD45RB-dim respective. Reacts with the 190, 205 and 220 kD isoforms of the cell-surface antigen CD45RB. CD45RB bright expression on T cells correlates with higher proliferation and IFN-g production in comparison to CD45RB dim expression. 90% of lymphocytes are CD45RB positive.
Flow Cytometry (20 ul/106 cells) The optimal working dilution should be determined by the end user.
In buffer containing 1% BSA, pH 7.2 (0.09% sodium azide)
Store in the dark at 4°C. Avoid prolonged exposure to light.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
CD45 antigen,T200 glycoprotein,T200 leukocyte common antigen,glycoprotein,leukocyte-common antigen,protein tyrosine phosphatase, receptor type, c polypeptide