KRT18 monoclonal antibody, clone C-04 (FITC)
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More Files
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Flow Cytometry
Intracellular flow cytometry analysis of KRT18 expression in HeLa (human cervix carcinoma cell line) using KRT18 monoclonal antibody, clone C-04 (FITC) (Cat # MAB4565).
Overlay with Isotype mouse IgG1 control.
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Specification
Product Description
Mouse monoclonal antibody raised against native KRT18.
Immunogen
Native purified KRT18 from cytoskeleton of epidermal carcinoma cell line A431.
Host
Mouse
Theoretical MW (kDa)
45
Reactivity
Human, Mammals
Specificity
This antibody reacts with Cytokeratin 18 (45 KDa).
Form
Liquid
Conjugation
FITC
Concentration
0.1 mg/mL
Isotype
IgG1
Recommend Usage
Flow Cytometry (10 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Intracellular flow cytometry analysis of KRT18 expression in HeLa (human cervix carcinoma cell line) using KRT18 monoclonal antibody, clone C-04 (FITC) (Cat # MAB4565).
Overlay with Isotype mouse IgG1 control. -
Gene Info — KRT18
Entrez GeneID
3875Gene Name
KRT18
Gene Alias
CYK18, K18
Gene Description
keratin 18
Gene Ontology
HyperlinkGene Summary
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
cell proliferation-inducing protein 46|cytokeratin 18
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Interactome
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Pathway
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Disease
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Publication Reference
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A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, Kovarík J.
The Journal of Pathology 1991 Jul; 164(3):215.
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Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule.
Lauerová L, Kovarik J, Bártek J, Rejthar A, Vojtĕsek B.
Hybridoma 1988 Oct; 7(5):495.
Application：IF, IHC-P, WB, Human, BT20 cells, Kidney, Liver, Lung, Oesophagus, Skin, Spleen, Stomach, Tongue.
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Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread.
Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J.
International Journal of Cancer. Supplement 1988 Jan; 3:50.
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A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
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