FAS monoclonal antibody, clone LT95 (PE)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Mouse monoclonal antibody raised against native FAS.
Immunogen
Native purified FAS from human T cell lymphoma cell line HUT-78.
Host
Mouse
Theoretical MW (kDa)
46
Reactivity
Human
Specificity
This antibody reacts with CD95 (Fas/APO-1), a 46 KDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
It seems that This antibody does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.Form
Liquid
Conjugation
PE
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Flow Cytometry
-
Gene Info — FAS
Entrez GeneID
355Gene Name
FAS
Gene Alias
ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6
Gene Description
Fas (TNF receptor superfamily, member 6)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq
Other Designations
APO-1 cell surface antigen|CD95 antigen|Fas AMA|Fas antigen|OTTHUMP00000020045|OTTHUMP00000020046|OTTHUMP00000020051|OTTHUMP00000059646|apoptosis antigen 1|tumor necrosis factor receptor superfamily member 6|tumor necrosis factor receptor superfamily, mem
-
Interactomes
-
Pathways
-
Diseases
-
Publication Reference
-
Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide.
Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP.
Experimental Cell Research 2008 Feb; 314(3):554.
Application:Flow Cyt, Human, CEM.neo, CEM.Bcl-2 cells.
-
Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines.
Guo Z, Zhang M, Tang H, Cao X.
Blood 2005 Sep; 106(6):2033.
-
Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway.
Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A.
The Journal of Biological Chemistry 2005 Jun; 280(24):22856.
Application:Flow Cyt, Human, HT-29 cells.
-
Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com