PTPRC monoclonal antibody, clone UCHL1 (PE)
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Specifications
Product Description
Mouse monoclonal antibody raised against native PTPRC.
Immunogen
Native purified PTPRC from human IL-2 dependent T cells.
Host
Mouse
Theoretical MW (kDa)
180
Reactivity
Human
Specificity
This antibody recognizes CD45R0, a 180 KDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.
Form
Liquid
Conjugation
PE
Isotype
IgG2a
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
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Gene Info — PTPRC
Entrez GeneID
5788Gene Name
PTPRC
Gene Alias
B220, CD45, CD45R, GP180, LCA, LY5, T200
Gene Description
protein tyrosine phosphatase, receptor type, C
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations
CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|glycoprotein|leukocyte-common antigen|protein tyrosine phosphatase, receptor type, c polypeptide
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.
Merkenschlager M, Terry L, Edwards R, Beverley PC.
European Journal of Immunology 1988 Nov; 18(11):1653.
Application:Flow Cyt, Human, Peripheral blood mononuclear cells.
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The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45.
Terry LA, Brown MH, Beverley PC.
Immunology 1988 Jun; 64(2):331.
Application:Flow Cyt, IP, Human, MOLT-4 cells.
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Phenotypic diversity of the CD45 antigen and its relationship to function.
Beverley PC, Merkenschlager M, Terry L.
Immunology. Supplement 1988 Jan; 1:3.
Application:Flow Cyt, Human, Mouse, Leucocytes.
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Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.
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