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Last updated: 2014/11/23
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CD44 monoclonal antibody, clone MEM-263

  • Catalog # : MAB3838
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native CD44.
  • Immunogen:
  • Native purified CD44 from African Green Monkey COS-7 cells.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 80-95
  • Reactivity:
  • Dog, Human, Pig
  • Specificity:
  • This antibody reacts with extracellular (N-terminal) domain of standard CD44(Phagocyte glycoprotein 1), a 80-95 KDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells,etc.); it is negative on platelets and hepatocytes.
  • Form:
  • Liquid
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (15 mM sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes (PBL) of various species using CD44 monoclonal antibody, clone MEM-263 (Cat # MAB3838).
    Lane 1 : Lysate of human PBL.
    Lane 2 : Lysate of canine PBL.
    Lane 3 : Lysate of porcine PBL.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of monoclonal antibody to CD44 on HepG2 cell. [antibody concentration 10 ug/ml]
  • Protocol Download
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of monoclonal antibody to CD44 on A-431 cell. [antibody concentration 10 ug/ml]
  • Protocol Download
  • Immunoprecipitation
  • Flow Cytometry
  • Immunofluorescence (Circulating Tumor Cell)
  • Immunofluorescence (Circulating Tumor Cell)
  • HepG2 cells were stained with CD44-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunoprecipitation
  • Flow Cytometry
  • Immunofluorescence (Circulating Tumor Cell)
  • Immunofluorescence (Circulating Tumor Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 960
  • Gene Name:
  • CD44
  • Gene Alias:
  • CDW44,CSPG8,ECMR-III,HCELL,IN,LHR,MC56,MDU2,MDU3,MGC10468,MIC4,MUTCH-I,Pgp1
  • Gene Description:
  • CD44 molecule (Indian blood group)
  • Gene Summary:
  • The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
  • Other Designations:
  • CD44 antigen,CD44 antigen (homing function and Indian blood group system),CDW44 antigen,GP90 lymphocyte homing/adhesion receptor,Hermes antigen,antigen gp90 homing receptor,cell adhesion molecule,cell surface glycoprotein CD44,chondroitin sulfate proteogl
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