CD44 monoclonal antibody, clone MEM-263
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes (PBL) of various species using CD44 monoclonal antibody, clone MEM-263 (Cat # MAB3838).
Lane 1 : Lysate of human PBL.
Lane 2 : Lysate of canine PBL.
Lane 3 : Lysate of porcine PBL.
Immunofluorescence (Circulating Tumor Cell)
HepG2 cells were stained with CD44-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to CD44 on A-431 cell. [antibody concentration 10 ug/ml]
Immunofluorescence
Immunofluorescence of monoclonal antibody to CD44 on HepG2 cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against native CD44.
Immunogen
Native purified CD44 from African Green Monkey COS-7 cells.
Host
Mouse
Theoretical MW (kDa)
80-95
Reactivity
Dog, Human, Pig
Specificity
This antibody reacts with extracellular (N-terminal) domain of standard CD44(Phagocyte glycoprotein 1), a 80-95 KDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells,etc.); it is negative on platelets and hepatocytes.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Western Blot (2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes (PBL) of various species using CD44 monoclonal antibody, clone MEM-263 (Cat # MAB3838).
Lane 1 : Lysate of human PBL.
Lane 2 : Lysate of canine PBL.
Lane 3 : Lysate of porcine PBL.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Flow Cytometry
Immunofluorescence (Circulating Tumor Cell)
HepG2 cells were stained with CD44-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to CD44 on A-431 cell. [antibody concentration 10 ug/ml]Immunofluorescence
Immunofluorescence of monoclonal antibody to CD44 on HepG2 cell. [antibody concentration 10 ug/ml] -
Gene Info — CD44
Entrez GeneID
960Gene Name
CD44
Gene Alias
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Gene Description
CD44 molecule (Indian blood group)
Omim ID
107269Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
Other Designations
CD44 antigen|CD44 antigen (homing function and Indian blood group system)|CDW44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|antigen gp90 homing receptor|cell adhesion molecule|cell surface glycoprotein CD44|chondroitin sulfate proteogl
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Interactome
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Pathway
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Disease
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Publication Reference
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Therapeutic anti-CD147 antibody sensitizes cells to chemoradiotherapy via targeting pancreatic cancer stem cells.
Fan XY, He D, Sheng CB, Wang B, Wang LJ, Wu XQ, Xu L, Jiang JL, Li L, Chen ZN.
American Journal of Translational Research 2019 Jun; 11(6):3543.
Application：WB-Ce, Human, MIA PaCa-2, PANC-1 cells.
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Genomic profiling of long non-coding RNA and mRNA expression associated with acquired temozolomide resistance in glioblastoma cells.
Zeng H, Xu N, Liu Y, Liu B, Yang Z, Fu Z, Lian C, Guo H.
International Journal of Oncology 2017 Jun; 51(2):445.
Application：IF, WB-Ce, Human, U87 MG cells.
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Dual role of CD44 isoforms in ampullary adenocarcinoma: CD44s predicts poor prognosis in early cancer and CD44ν is an indicator for recurrence in advanced cancer.
Wu CL, Chao YJ, Yang TM, Chen YL, Chang KC, Hsu HP, Shan YS, Lai MD.
BMC Cancer 2015 Nov; 15:903.
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Concurrent CD44s and STAT3 expression in human clear cell renal cellular carcinoma and its impact on survival.
Qin J, Yang B, Xu BQ, Smithc A, Xu L, Yuan JL, Li L.
International Journal of Clinical and Experimental Pathology 2014 May; 7(6):3235.
Application：IHC-P, Human, Renal cell carcinoma.
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HAb18G/CD147 Promotes pSTAT3-Mediated Pancreatic Cancer Development via CD44s.
Li L, Tang W, Karnak D, Wu X, Meng X, Lefferdink RL, Hao X, Li Y, Qiao X, Lin J, Fuchs JR, Simeone DM, Chen Z, Lawrence TS, Xu L.
Clinical Cancer Research 2013 Dec; 19(24):6703.
Application：IF, IHC-P, WB-Tr, Human, PANC-1, HEK 293, MIA PaCa-2 cells, Pancreas.
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Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells.
Subramaniam V, Gardner H, Jothy S.
Experimental and Molecular Pathology 2007 Dec; 83(3):341.
Application：WB-Ce, Human, SW620 cells.
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CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation.
Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S.
Experimental and Molecular Pathology 2007 May; 83(2):207.
Application：IP-WB, Human, HT29, SW620 cells.
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Therapeutic anti-CD147 antibody sensitizes cells to chemoradiotherapy via targeting pancreatic cancer stem cells.
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