Mouse monoclonal antibody raised against native CD34.
Native purified CD34 from permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Theoretical MW (kDa):
This antibody reacts with Class III epitope on CD34 (Mucosialin), a 110-115 KDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Flow Cytometry (2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL) Western Blot (2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (15 mM sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.