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Last updated: 2014/9/1
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PRKDC monoclonal antibody, clone 18-2, 25-4, 42-psc

  • Catalog # : MAB3433
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native PRKDC.
  • Immunogen:
  • Native purified PRKDC from HeLa cells.
  • Host:
  • Mouse
  • Reactivity:
  • Chicken, Clawed frog, Hamster, Human, Mouse
  • Specificity:
  • Reacts with the nuclear catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). Epitopes of the three clones have not been precisely mapped, but experimental data suggests that they are different.
  • Form:
  • Liquid
  • Isotype:
  • IgG1; IgG2a; IgG1
  • Recommend Usage:
  • Immunoprecipitation (2 ug/mg protein lysate)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunofluorescence
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation/Western blot analysis of PRKDC expression in LS174T and HUVEC cells. Immunoprecipitation (native and denatured) using 2 ul/mg protein lysate. Use Protein G.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 5591
  • Gene Name:
  • PRKDC
  • Gene Alias:
  • DNA-PKcs,DNAPK,DNPK1,HYRC,HYRC1,XRCC7,p350
  • Gene Description:
  • protein kinase, DNA-activated, catalytic polypeptide
  • Gene Summary:
  • The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
  • Other Designations:
  • hyper-radiosensitivity of murine scid mutation, complementing 1
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