Mouse monoclonal antibody raised against native PRKDC.
Native purified PRKDC from HeLa cells.
Reacts with the 460 KDa catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). Inhibits kinase activity of DNA-PKcs ~50%, but preincubation of enzyme with DNA protects DNA-PKcs from inactivation by clone 18-2. In Western blot, clone 18-2 reacts with intact DNA-PKcs as well as its degradation products.
Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
In 10 mM PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
hyper-radiosensitivity of murine scid mutation, complementing 1