PRKDC monoclonal antibody, clone 18-2
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Specification
Product Description
Mouse monoclonal antibody raised against native PRKDC.
Immunogen
Native purified PRKDC from HeLa cells.
Host
Mouse
Reactivity
Human, Rat
Specificity
Reacts with the 460 KDa catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). Inhibits kinase activity of DNA-PKcs ~50%, but preincubation of enzyme with DNA protects DNA-PKcs from inactivation by clone 18-2. In Western blot, clone 18-2 reacts with intact DNA-PKcs as well as its degradation products.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Immunoprecipitation
Immunoprecipitation/Western blot analysis of PRKDC expression in LS174T cells. Immunoprecipitation (native and denatured) using 2 ul/mg protein lysate. Use Protein G. -
Gene Info — PRKDC
Entrez GeneID
5591Gene Name
PRKDC
Gene Alias
DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, XRCC7, p350
Gene Description
protein kinase, DNA-activated, catalytic polypeptide
Omim ID
600899Gene Ontology
HyperlinkGene Summary
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
Other Designations
hyper-radiosensitivity of murine scid mutation, complementing 1
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