Il6 monoclonal antibody, clone MP5-20F3
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Specifications
Product Description
Rat monoclonal antibody raised against recombinant Il6.
Immunogen
Recombinant protein corresponding to mouse Il6.
Host
Rat
Reactivity
Mouse
Specificity
Mouse Interleukin-6.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Western Blot
Immunocytochemistry
Flow Cytometry
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Gene Info — Il6
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Publication Reference
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Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies.
Prussin C, Metcalfe DD.
Journal of Immunological Methods 1995 Dec; 188(1):117.
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Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
B Sander, I Höidén, U Andersson, E Möller, J S Abrams.
Journal of Immunological Methods 1993 Dec; 166(2):201.
Application:EIA, IF, Mouse, T cells, T cells culture supernatants.
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Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE.
Immunological Reviews 2006 Jun; 127:5.
Application:Blocking, ELISA, Mouse, Supernatants from cells cultured and stimulated ex vivo with different agents and biological fluids like sera/plasma, bronehoalveolar lavage, ascites, urines, cerebrospinal fluids, saliva, and sputa. .
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Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies.
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