Itgam monoclonal antibody, clone M1/70
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More Files
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Flow Cytometry
BALB/c splenocytes were double stained with Itgam monoclonal antibody, clone M1/70 (Cat # MAB3224)-FITC and rat anti-mouse Ly-6G-PE. Lymphocytes were gated and analyzed by flow cytometry.
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Specification
Product Description
Rat monoclonal antibody raised against Itgam.
Immunogen
Native Itgam from mouse splenic T cells and splenocytes.
Host
Rat
Reactivity
Mouse
Specificity
alphaM subunit (Mr 170 KDa) of the CD11b/CD18 heterodimer.
Form
Liquid
Isotype
IgG2b
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c splenocytes were double stained with Itgam monoclonal antibody, clone M1/70 (Cat # MAB3224)-FITC and rat anti-mouse Ly-6G-PE. Lymphocytes were gated and analyzed by flow cytometry. -
Gene Info — Itgam
Entrez GeneID
16409Gene Name
Itgam
Gene Alias
CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a
Gene Description
integrin alpha M
Gene Ontology
HyperlinkOther Designations
CD11B (p170)|Mac-1 alpha|cell surface glycoprotein MAC-1 alpha subunit|complement component receptor 3 alpha|complement component receptor 3 alpha-a|complement receptor type 3|leukocyte adhesion receptor MO1|macrophage antigen alpha
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Publication Reference
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Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection.
Gyetko MR, Sud S, Kendall T, Fuller JA, Newstead MW, Standiford TJ.
Journal of Immunology 2000 Aug; 165(3):1513.
Application：Func, Mouse, Neutrophils present in bronchoalveolar lavage fluids.
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Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54).
Lub M, van Kooyk Y, Figdor CG.
Journal of Leukocyte Biology 1996 May; 59(5):648.
Application：IP, Mouse, Mouse macrophages.
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Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm.
Springer TA.
Cell 1994 Jan; 76(2):301.
Application：Flow Cyt, Human, Leukocytes.
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Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection.
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