RBP4 monoclonal antibody, clone AT2B4
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HepG2 cell lysate, Lane 2: 293T cell lysate, Lane 3: RBP4 Transfected 293T cell lysate.
Immunofluorescence
Immunofluorescence analysis of Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal RBP4 monoclonal antibody, clone AT2B4 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry
Flow cytometric analysis of HepG2 cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant RBP4.
Immunogen
Recombinant protein corresponding to amino acids 19-201 of human RBP4.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HepG2 cell lysate, Lane 2: 293T cell lysate, Lane 3: RBP4 Transfected 293T cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal RBP4 monoclonal antibody, clone AT2B4 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — RBP4
Entrez GeneID
5950Protein Accession#
NP_006735Gene Name
RBP4
Gene Alias
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Gene Description
retinol binding protein 4, plasma
Omim ID
180250Gene Ontology
HyperlinkGene Summary
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq
Other Designations
OTTHUMP00000020114|OTTHUMP00000020116|retinol-binding protein 4, interstitial|retinol-binding protein 4, plasma
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Interactome
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Disease
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Publication Reference
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Retinol binding protein IV purified from Escherichia coli using intein-mediated cleavage as a suitable replacement for serum sources.
Est CB, Murphy RM.
Protein Expression and Purification 2020 Mar; 167:105542.
Application：WB-Re, Recombinant protein.
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Increased retinol-binding protein 4 (RBP4) and anti-RBP4 antibody in alopecia areata.
Ahn KJ, Choi EA, Kim J, Lee JH, Lee KH, Bang D, Cho SB.
The British Journal of Dermatology 2011 Oct; 165(4):837.
Application：IHC-P, Human, Alopecia areata.
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Editorial: Retinol binding protein 4 and pathogenesis of diabetes.
McTernan PG, Kumar S.
The Journal of Clinical Endocrinology and Metabolism 2007 Jul; 92(7):2430.
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Retinol binding protein 4 expression in humans: relationship to insulin resistance, inflammation, and response to pioglitazone.
Yao-Borengasser A, Varma V, Bodles AM, Rasouli N, Phanavanh B, Lee MJ, Starks T, Kern LM, Spencer HJ 3rd, Rashidi AA, McGehee RE Jr, Fried SK, Kern PA.
The Journal of Clinical Endocrinology and Metabolism 2007 Jul; 92(7):2590.
Application：ELISA, WB-Ti, Human, Human adipose tissue.
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Retinol binding protein IV purified from Escherichia coli using intein-mediated cleavage as a suitable replacement for serum sources.
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