Immunoblot analysis of cell lysates. Lane 1 : Bosc23 cell lysate. Lane 2 : Bosc23 cell lysate transfected with Myc/His/Tau (1-441 a. a.). Lane 3 : Bosc23 cell lysate transfected with Myc/His/CDC25A (291-524E a. a.). Lane 4 : Bosc23 cell lysate transfected with GFP-Ubiquitin+1.
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq