Mouse monoclonal antibody raised against synthetic peptide of MMP2.
A synthetic peptide corresponding to N-terminus of human MMP2.
Theoretical MW (kDa):
Human, Mouse, Rat
This antibody recognizes ~72 KDa of MMP-2 pro form (procollagenase IV).
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (2-5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (10% Proclin300)
Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
Immunohistochemical staining of human placenta stained with MMP2 monoclonal antibody, clone SPM346 (Cat # MAB2541) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
collagenase type IV-A,matrix metalloproteinase 2,matrix metalloproteinase-II,neutrophil gelatinase