KRT18 monoclonal antibody, clone SPM265
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer stained with KRT18 monoclonal antibody, clone SPM265 (Cat # MAB2540) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
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Specification
Product Description
Mouse monoclonal antibody raised against native KRT18.
Immunogen
Native KRT18 from human breast cancer PMC 42 cells.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes human KRT18,
Form
Liquid
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Western Blot (0.1-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50: 2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer stained with KRT18 monoclonal antibody, clone SPM265 (Cat # MAB2540) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.Immunofluorescence
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Gene Info — KRT18
Entrez GeneID
3875Gene Name
KRT18
Gene Alias
CYK18, K18
Gene Description
keratin 18
Gene Ontology
HyperlinkGene Summary
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
cell proliferation-inducing protein 46|cytokeratin 18
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Interactome
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Pathway
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Disease
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