APEX1 monoclonal antibody, clone 13B8E5C2
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Specification
Product Description
Mouse monoclonal antibody raised against APEX1.
Immunogen
Human APEX1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to the human APE/ref-1 protein.
Form
Liquid
Isotype
IgG2b
Quality Control Testing
Antibody Reactive Against APEX1.
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of APEX1 on Ovarian Cancer cell lines using APEX1 monoclonal antibody, clone 13B8E5C2 (Cat # MAB2372).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — APEX1
Entrez GeneID
328Protein Accession#
P27695Gene Name
APEX1
Gene Alias
APE, APE-1, APE1, APEN, APEX, APX, HAP1, REF-1, REF1
Gene Description
APEX nuclease (multifunctional DNA repair enzyme) 1
Omim ID
107748Gene Ontology
HyperlinkGene Summary
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq
Other Designations
AP endonuclease class I|AP lyase|APEX nuclease|DNA-(apurinic or apyrimidinic site) lyase|OTTHUMP00000163988|apurinic/apyrimidinic (abasic) endonuclease|apurinic/apyrimidinic exonuclease|deoxyribonuclease (apurinic or apyrimidinic)|multifunctional DNA repa
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Interactome
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Pathway
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Disease
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Publication Reference
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Regulatory role of human AP-endonuclease (APE1/Ref-1) in YB-1-mediated activation of the multidrug resistance gene MDR1.
Chattopadhyay R, Das S, Maiti AK, Boldogh I, Xie J, Hazra TK, Kohno K, Mitra S, Bhakat KK.
Molecular and Cellular Biology 2008 Dec; 28(23):7066.
Application:WB, Human, HCT-116, MCF-7 cells.
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Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres.
May A, Kirchner R, Muller H, Hartmann P, El Hajj N, Tresch A, Zechner U, Mann W, Haaf T.
Biology of Reproduction 2009 Jan; 80(1):194.
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The impact of hypoxic treatment on the expression of phosphoglycerate kinase and the cytotoxicity of troxacitabine and gemcitabine.
Lam W, Leung CH, Bussom S, Cheng YC.
Molecular Pharmacology 2007 Sep; 72(3):536.
Application:WB-Ce, Human, A673, HepG2, Hep3B, Panc-1 cells.
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Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways.
Kundu CN, Balusu R, Jaiswal AS, Narayan S.
Carcinogenesis 2007 Oct; 28(10):2089.
Application:WB, Mouse, MEFs.
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Apurinic/apyrimidinic endonuclease-1 protein level is associated with the cytotoxicity of L-configuration deoxycytidine analogs (troxacitabine and beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluorocytidine) but not D-configuration deoxycytidine analogs (gemcitabine and beta-D-arabinofuranosylcytosine).
Lam W, Park SY, Leung CH, Cheng YC.
Molecular Pharmacology 2006 May; 69(5):1607.
Application:IF, WB, Human, RKO cells.
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Regulatory role of human AP-endonuclease (APE1/Ref-1) in YB-1-mediated activation of the multidrug resistance gene MDR1.
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