Mouse monoclonal antibody raised against recombinant MMP2.
Recombinant protein corresponding to human MMP2.
Human, Mouse, Rat
This antibody is specific to pro and active MMP2.
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
ELISA (0.3 ug/mL) Western Blot (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2 ug/mL) Immunohistochemistry (Frozen sections) (2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (0.05% sodium azide)
Store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Detection of MMP2 staining in formalin-fixed paraffin-embedded human ovary tissue using MMP2 monoclonal antibody, clone MMP2/8B4 (Cat # MAB2335). Primary antibody was used at 2.5 ug/mL with the avidin-biotin-peroxidase detection method.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
collagenase type IV-A,matrix metalloproteinase 2,matrix metalloproteinase-II,neutrophil gelatinase