FABP4 monoclonal antibody, clone 3F4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FABP4.
Immunogen
Recombinant protein corresponding to amino acids 1-132 of human FABP4.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse heart tissue lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with FABP4 monoclonal antibody, clone 3F4 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — FABP4
Entrez GeneID
2167Protein Accession#
NP_001433Gene Name
FABP4
Gene Alias
A-FABP, aP2
Gene Description
fatty acid binding protein 4, adipocyte
Omim ID
600434Gene Ontology
HyperlinkGene Summary
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2.
Furuhashi M, Tuncman G, Gorgun CZ, Makowski L, Atsumi G, Vaillancourt E, Kono K, Babaev VR, Fazio S, Linton MF, Sulsky R, Robl JA, Parker RA, Hotamisligil GS.
Nature 2007 Jun; 447(7147):959.
Application:WB-Ti, Mouse, Mouse adipose tissues.
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The adipocyte fatty acid-binding protein aP2 is required in allergic airway inflammation.
Shum BO, Mackay CR, Gorgun CZ, Frost MJ, Kumar RK, Hotamisligil GS, Rolph MS.
The Journal of Clinical Investigation 2006 Aug; 116(8):2183.
Application:IHC-P, Mouse, Mouse lung.
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Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2.
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