The recombinant fusion proteins and cell lysate were resolved by SDS-PAGE and probed with CTSL1 monoclonal antibody, clone 2H7 (Cat # MAB2047) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : CTSB. Lane 2 : CTSD. Lane 3 : CTSK. Lane 4 : CTSL1. Lane 5 : CTSS. Lane 6 : A-549.
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
OTTHUMP00000021601,OTTHUMP00000021602,OTTHUMP00000063566,cathepsin L,major excreted protein