HSPH1 monoclonal antibody, clone J1G12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: NIH-3T3 cell lysate, Lane 4: MCF7 cell lysate, Lane 5: U-87 MG cell lysate, Lane 6: A549 cell lysate.
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with HSPH1 monoclonal antibody, clone J1G12 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HSPH1.
Immunogen
Recombinant protein corresponding to amino acids 1-858 of human HSPH1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: NIH-3T3 cell lysate, Lane 4: MCF7 cell lysate, Lane 5: U-87 MG cell lysate, Lane 6: A549 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with HSPH1 monoclonal antibody, clone J1G12 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — HSPH1
Entrez GeneID
10808Protein Accession#
Q92598Gene Name
HSPH1
Gene Alias
DKFZp686M05240, HSP105, HSP105A, HSP105B, KIAA0201, NY-CO-25
Gene Description
heat shock 105kDa/110kDa protein 1
Omim ID
610703Gene Ontology
HyperlinkOther Designations
OTTHUMP00000018208|heat shock 105kD|heat shock 105kD alpha|heat shock 105kD beta|heat shock 105kDa protein 1
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Interactome
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Publication Reference
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Different localization of Hsp105 family proteins in mammalian cells.
Saito Y, Yamagishi N, Hatayama T.
Experimental Cell Research 2007 Oct; 313(17):3707.
Application：IF, WB-Tr, Monkey, COS-7 cells.
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Hsp105 family proteins suppress staurosporine-induced apoptosis by inhibiting the translocation of Bax to mitochondria in HeLa cells.
Yamagishi N, Ishihara K, Saito Y, Hatayama T.
Experimental Cell Research 2006 Oct; 312(17):3215.
Application：WB-Ce, Human, HeLa-tet/Hsp105α, HeLa-tet/Hsp105β cells.
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Characterization of heat shock protein 110 and glucose-regulated protein 170 as cancer vaccines and the effect of fever-range hyperthermia on vaccine activity.
Wang XY, Kazim L, Repasky EA, Subjeck JR.
The Journal of Immunology 2001 Jan; 166(1):490.
Application：WB-Ti, Mouse, Colon tumor, Liver.
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Different localization of Hsp105 family proteins in mammalian cells.
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