CDC2 monoclonal antibody, clone POH-1
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More Files
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Western Blot (Cell lysate)
CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) is shown to detect human CDC2 by western blot.
Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane.
The blot was incubated with a 1:1,000 dilution of CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000.
A doublet band corresponding to human CDC2 is detected at ~34 KDa when compared with known molecular weight standards (not shown).
The antibody may be used to detect endogenous human CDC2.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
Western Blot (Cell lysate)
CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) was used to detect human CDC2 by western blot in untreated (control) and drug treated (10 uM genistein) lysates of MCF-7 cells.
Very strong detection occurs using a 1:1,000 dilution.
Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant CDC2.
Immunogen
Recombinant protein corresponding to human CDC2.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases.
Form
Liquid
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 500 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) is shown to detect human CDC2 by western blot.
Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane.
The blot was incubated with a 1:1,000 dilution of CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000.
A doublet band corresponding to human CDC2 is detected at ~34 KDa when compared with known molecular weight standards (not shown).
The antibody may be used to detect endogenous human CDC2.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.Western Blot (Cell lysate)
CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) was used to detect human CDC2 by western blot in untreated (control) and drug treated (10 uM genistein) lysates of MCF-7 cells.
Very strong detection occurs using a 1:1,000 dilution.
Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC2
Entrez GeneID
983Gene Name
CDC2
Gene Alias
CDC28A, CDK1, DKFZp686L20222, MGC111195
Gene Description
cell division cycle 2, G1 to S and G2 to M
Omim ID
116940Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019660|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2 protein|cyclin-dependent kinase 1|p34 protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
Palmer A, Gavin AC, Nebreda AR.
The EMBO Journal 1998 Sep; 17(17):5037.
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Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2.
Ye XS, Fincher RR, Tang A, McNeal KK, Gygax SE, Wexler AN, Ryan KB, James SW, Osmani SA.
The Journal of Biological Chemistry 1997 Dec; 272(52):33384.
Application：IP, WB-Ce, WB-Tr, Yeast, Aspergillus cells.
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Distinct forms of human CDC2 identified by novel monoclonal antibodies.
Lukas J, Draetta G, Bartek J.
European Journal of Biochemistry 1992 Jul; 207(1):169.
Application：IP, WB-Re, Human, HeLa cells, Recombinant proteins.
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A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
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