HSPB1 monoclonal antibody, clone G3.1
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining using HSPB1 monoclonal antibody, clone G3.1 on formalin-fixed, paraffin-embedded human breast carcinoma with peroxidase-conjugate and DAB chromogen.
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Specification
Product Description
Mouse monoclonal antibody raised against native HSPB1.
Immunogen
Native purified human HSPB1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining using HSPB1 monoclonal antibody, clone G3.1 on formalin-fixed, paraffin-embedded human breast carcinoma with peroxidase-conjugate and DAB chromogen.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSPB1
Entrez GeneID
3315Gene Name
HSPB1
Gene Alias
CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Gene Description
heat shock 27kDa protein 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq
Other Designations
OTTHUMP00000024846|estrogen-regulated 24 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27
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Interactome
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Pathway
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Disease
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Publication Reference
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Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.
Hino M, Kurogi K, Okubo MA, Murata-Hori M, Hosoya H.
Biochemical and Biophysical Research Communications 2000 Apr; 271(1):164.
Application：IF, IP, WB, Human, HeLa cells, Recombinant protein.
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Immunological evidence for the identity between the hsp27 estrogen-regulated heat shock protein and the p29 estrogen receptor-associated protein in breast and endometrial cancer.
Ciocca DR, Luque EH.
Breast Cancer Research and Treatment 1991 Dec; 20(1):33.
Application：IHC-P, IP, WB-Ce, WB-Ti, Human, Human breast cancer, Human endometrial cancer, MCF-7 cells.
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A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells.
Moretti-Rojas I, Fuqua SA, Montgomery RA 3rd, McGuire WL.
Breast Cancer Research and Treatment 1988 May; 11(2):155.
Application：IP, Human, Rabbit, MCF-7 cells, Rabbit reticulocytes.
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Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.
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