PGR (phospho S294) monoclonal antibody, clone 608
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of whole cell T-47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PGR-A isoform and the ~120 PGR-B isoform of the PGR phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of PGR.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S294 of human PGR.
Host
Mouse
Theoretical MW (kDa)
90, 120
Reactivity
Human, Mouse, Primates, Rat
Specificity
This antibody recognizes ~90KDa PR-A isoform and the ~120 KDa PR-B isoform.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (Frozen sections) (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of whole cell T-47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PGR-A isoform and the ~120 PGR-B isoform of the PGR phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).Immunohistochemistry (Frozen sections)
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Gene Info — PGR
Entrez GeneID
5241Protein Accession#
P06401Gene Name
PGR
Gene Alias
NR3C3, PR
Gene Description
progesterone receptor
Omim ID
607311Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform B has not been clearly determined. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Regulation of signal transduction pathways by estrogen and progesterone.
Edwards DP.
Annual Review of Physiology 2005 Jan; 67:335.
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Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion.
Lin VC, Woon CT, Aw SE, Guo C.
Endocrinology 2003 Dec; 144(12):5650.
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Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody.
Clemm DL, Sherman L, Boonyaratanakornkit V, Schrader WT, Weigel NL, Edwards DP.
Molecular Endocrinology 2000 Jan; 14(1):52.
Application：WB-Ce, Human, T47D cells.
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Regulation of signal transduction pathways by estrogen and progesterone.
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