ACOT11 monoclonal antibody, clone J4B2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: LNCap cell lysate, Lane 2: Hep3B cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon cancer tissue were incubated with ACOT11 monoclonal antibody, clone J4B2 (Cat # MAB1770) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ACOT11.
Immunogen
Recombinant protein corresponding to amino acids 19-250 of human ACOT11.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: LNCap cell lysate, Lane 2: Hep3B cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon cancer tissue were incubated with ACOT11 monoclonal antibody, clone J4B2 (Cat # MAB1770) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACOT11
Entrez GeneID
26027Protein Accession#
Q8WXI4Gene Name
ACOT11
Gene Alias
BFIT, BFIT1, BFIT2, KIAA0707, STARD14, THEA, THEM1
Gene Description
acyl-CoA thioesterase 11
Omim ID
606803Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of a similar murine protein in brown adipose tissue is induced by cold exposure and repressed by warmth. Expression of the mouse protein has been associated with obesity, with higher expression found in obesity-resistant mice compared with obesity-prone mice. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000011526|OTTHUMP00000011527|OTTHUMP00000046722|START domain containing 14|StAR-related lipid transfer (START) domain containing 14|brown fat inducible thioesterase|thioesterase superfamily member 1|thioesterase, adipose associated
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Interactome
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Disease
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Publication Reference
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BFIT, a unique acyl-CoA thioesterase induced in thermogenic brown adipose tissue: cloning, organization of the human gene and assessment of a potential link to obesity.
Adams SH, Chui C, Schilbach SL, Yu XX, Goddard AD, Grimaldi JC, Lee J, Dowd P, Colman S, Lewin DA.
The Biochemical Journal 2001 Nov; 360(Pt 1):135.
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BFIT, a unique acyl-CoA thioesterase induced in thermogenic brown adipose tissue: cloning, organization of the human gene and assessment of a potential link to obesity.
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