MAPK1/MAPK3 (phospho T202/204) monoclonal antibody, clone G15-B
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More Files
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Western Blot
MAPK1/MAPK3 (phospho T202/204) monoclonal antibody, clone G15-B (Cat # MAB1637). Western blot of MAPK1/MAPK3 activation in serum free DMEM, and EGF-100 ng/mL , 5min with 10% FBS in DMEM (4). Wells were equally loaded with 50 ug of whole cell lysate proteins/well. Western blot was performed and kindly provided by Dr. Martina Takacova from Intitute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia.
Immunofluorescence
Immunofluorescence staining of HEK293 cells (A, untreated) and hydrogen peroxide treated cells (B) with MAPK1/MAPK3 (phospho T202/204) monoclonal antibody, clone G15-B (Cat # MAB1637) at 1:100 dilution.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of MAPK1/MAPK3.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T202/T204 of human MAPK1/MAPK3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000-1:100000)
Immunofluorescence
Immunoprecipitation
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, pH 8.0 (10 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
MAPK1/MAPK3 (phospho T202/204) monoclonal antibody, clone G15-B (Cat # MAB1637). Western blot of MAPK1/MAPK3 activation in serum free DMEM, and EGF-100 ng/mL , 5min with 10% FBS in DMEM (4). Wells were equally loaded with 50 ug of whole cell lysate proteins/well. Western blot was performed and kindly provided by Dr. Martina Takacova from Intitute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia.Immunofluorescence
Immunofluorescence staining of HEK293 cells (A, untreated) and hydrogen peroxide treated cells (B) with MAPK1/MAPK3 (phospho T202/204) monoclonal antibody, clone G15-B (Cat # MAB1637) at 1:100 dilution.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK1
Entrez GeneID
5594Gene Name
MAPK1
Gene Alias
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Gene Description
mitogen-activated protein kinase 1
Omim ID
176948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174492|extracellular signal-regulated kinase 2|extracellular signal-regulated kinase-2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
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Gene Info — MAPK3
Entrez GeneID
5595Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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REST and Neural Gene Network Dysregulation in iPSC Models of Alzheimer's Disease.
Meyer K, Feldman HM, Lu T, Drake D, Lim ET, Ling KH, Bishop NA, Pan Y, Seo J, Lin YT, Su SC, Church GM, Tsai LH, Yankner BA.
Cell Reports 2019 Jan; 26(5):1112.
Application：WB, Human, Human dermal fibroblast cells.
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Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts.
Lee WR, Shen SC, Shih YH, Chou CL, Tseng JT, Chin SY, Liu KH, Chen YC, Jiang MC.
Journal of Translational Medicine 2015 Jun; 13:191.
Application：WB-Ce, Human, B16-dEV, B16-CSE1L, B16-Ras cells.
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CSE1L Links cAMP/PKA and Ras/ERK pathways and regulates the expressions and phosphorylations of ERK1/2, CREB, and MITF in melanoma cells.
Lee WR, Shen SC, Wu PR, Chou CL, Shih YH, Yeh CM, Yeh KT, Jiang MC.
Molecular Carcinogenesis 2016 Nov; 55(11):1542.
Application：WB, Mouse, B16F10 mouse melanoma cells.
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High nuclear expression of phosphorylated extracellular signal-regulated kinase in tumor cells in colorectal glands is associated with poor outcome in colorectal cancer.
Tai CJ, Lee CH, Chen HC, Wang HK, Jiang MC, Su TC, Shen KH, Lin SH, Yeh CM, Chen CJ, Yeh KT, Chang CC.
Annals of Diagnostic Pathology 2013 Apr; 17(2):165.
Application：IHC-P, Human, Colorectal cancer.
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Clinical-pathological correlation of K-Ras mutation and ERK phosphorylation in colorectal cancer.
Tai CJ, Chang CC, Jiang MC, Yeh CM, Su TC, Wu PR, Chen CJ, Yeh KT, Lin SH, Chen HC.
Polish Journal of Pathology 2012 Jun; 63(2):93.
Application：IHC-P, Human, Human colon cancer.
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REST and Neural Gene Network Dysregulation in iPSC Models of Alzheimer's Disease.
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