DMD monoclonal antibody, clone MANDYS8
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant DMD.
Immunogen
Recombinant protein corresponding to full length human DMD.
Host
Mouse
Theoretical MW (kDa)
426.6
Reactivity
Chicken, Human, Mouse, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG2b
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 1.2% sodium acetate (2 mg BSA, 0.01 mg sodium azide)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
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Gene Info — DMD
Entrez GeneID
1756Gene Name
DMD
Gene Alias
BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272
Gene Description
dystrophin
Gene Ontology
HyperlinkGene Summary
The dystrophin gene is the largest gene found in nature, measuring 2.4 Mb. The gene was identified through a positional cloning approach, targeted at the isolation of the gene responsible for Duchenne (DMD) and Becker (BMD) Muscular Dystrophies. DMD is a recessive, fatal, X-linked disorder occurring at a frequency of about 1 in 3,500 new-born males. BMD is a milder allelic form. In general, DMD patients carry mutations which cause premature translation termination (nonsense or frame shift mutations), while in BMD patients dystrophin is reduced either in molecular weight (derived from in-frame deletions) or in expression level. The dystrophin gene is highly complex, containing at least eight independent, tissue-specific promoters and two polyA-addition sites. Furthermore, dystrophin RNA is differentially spliced, producing a range of different transcripts, encoding a large set of protein isoforms. Dystrophin (as encoded by the Dp427 transcripts) is a large, rod-like cytoskeletal protein which is found at the inner surface of muscle fibers. Dystrophin is part of the dystrophin-glycoprotein complex (DGC), which bridges the inner cytoskeleton (F-actin) and the extra-cellular matrix. [provided by RefSeq
Other Designations
Duchenne muscular dystrophy protein|OTTHUMP00000023117|OTTHUMP00000023124|OTTHUMP00000023125|OTTHUMP00000023126|muscular dystrophy, Duchenne and Becker types
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Interactome
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Pathway
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Disease
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Publication Reference
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Prime editing strategies to mediate exon skipping in DMD gene.
Cedric Happi Mbakam, Jeanne Roustant, Joel Rousseau, Pouire Yameogo, Yaoyao Lu, Anne Bigot, Kamel Mamchaoui, Vincent Mouly, Gabriel Lamothe, Jacques P Tremblay.
Frontiers in nuclear medicine (Lausanne, Switzerland) 2023 May; 10:1128557.
Application:WB-Ce, Human, Human myoblasts.
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Prime editing optimized RTT permits the correction of the c.8713C>T mutation in DMD gene.
Cedric Happi Mbakam, Joel Rousseau, Yaoyao Lu, Anne Bigot, Kamel Mamchaoui, Vincent Mouly, Jacques P Tremblay.
Molecular Therapy. Nucleic Acids 2022 Dec; 30:272.
Application:WB-Ce, Human, Human myoblasts.
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Serum extracellular vesicles for delivery of CRISPR-CAS9 ribonucleoproteins to modify the dystrophin gene.
Nathalie Majeau, Annabelle Fortin-Archambault, Catherine Gérard, Joël Rousseau, Pouiré Yaméogo, Jacques P Tremblay.
Molecular Therapy 2022 Jul; 30(7):2429.
Application:IF, Mouse, Muscle.
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Overexpression of dystrophin in transgenic mdx mice eliminates dystrophic symptoms without toxicity.
Cox GA, Cole NM, Matsumura K, Phelps SF, Hauschka SD, Campbell KP, Faulkner JA, Chamberlain JS.
Nature 1993 Aug; 364(6439):725.
Application:WB-Ti, WB-Tr, Mouse, Skeletal muscle.
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Dystrophin gene transcribed from different promoters in neuronal and glial cells.
Chelly J, Hamard G, Koulakoff A, Kaplan JC, Kahn A, Berwald-Netter Y.
Nature 1990 Mar; 344(6261):64.
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Prime editing strategies to mediate exon skipping in DMD gene.
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