GSK3B (phospho Y216) monoclonal antibody, clone M132
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of GSK3B.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y216 of human GSK3B.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This peptide sequence is also found in GSK-3a (Tyr-279) and is highly conserved in GSK-3 genes in rat and mouse.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lane 1) or treated with pervanadate (1 mM) for 30 minutes (lane 2). The blot was probed with GSK3B (phospho Y216) monoclonal antibody, clone M132 (Cat # MAB1373).Enzyme-linked Immunoabsorbent Assay
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Gene Info — GSK3B
Entrez GeneID
2932Gene Name
GSK3B
Gene Alias
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Gene Description
glycogen synthase kinase 3 beta
Omim ID
605004Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
Other Designations
GSK3beta isoform|glycogen synthase kinase-3 beta
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Interactome
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Pathway
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Disease
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Publication Reference
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Retinoic acid attenuates beta-amyloid deposition and rescues memory deficits in an Alzheimer's disease transgenic mouse model.
Ding Y, Qiao A, Wang Z, Goodwin JS, Lee ES, Block ML, Allsbrook M, McDonald MP, Fan GH.
Journal of Neuroscience 2008 Nov; 28(45):11622.
Application:WB-Ti, Mouse, Mouse cortical and hippocampal lysates.
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Glycogen synthase kinase-3beta is tyrosine-phosphorylated by MEK1 in human skin fibroblasts.
Takahashi-Yanaga F, Shiraishi F, Hirata M, Miwa Y, Morimoto S, Sasaguri T.
Biochemical and Biophysical Research Communications 2004 Apr; 316(2):411.
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Glycogen synthase kinase-3beta suppression eliminates tumor necrosis factor-related apoptosis-inducing ligand resistance in prostate cancer.
Liao X, Zhang L, Thrasher JB, Du J, Li B.
Molecular Cancer Therapeutics 2003 Nov; 2(11):1215.
Application:WB-Ce, Human, DU145 cells.
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Retinoic acid attenuates beta-amyloid deposition and rescues memory deficits in an Alzheimer's disease transgenic mouse model.
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