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CTNNB1 monoclonal antibody, clone M118   

  • Catalog # : MAB1369
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  • 100 uL
  • USD $ 319
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CTNNB1.
  • Immunogen:
  • Recombinant protein corresponding to C-terminus of human CTNNB1.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This peptide sequence is highly conserved in rat and mouse beta-Catenin.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:2000)
    Immunocytochemistry (1:250)
    Immunoprecipitation (5-10 ul)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of CTNNB1 immunoprecipitated from A-431 cells treated with pervanadate. The blots were probed with CTNNB1 monoclonal antibody, clone M118 (Cat # MAB1369). The immunoprecipitated CTNNB1 was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin fixed, citric acid treated parafin sections of embryonic rat E16 intestines. Sections were probed with CTNNB1 monoclonal antibody, clone M118 (Cat # MAB1369) then anti-mouse HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1499
  • Gene Name:
  • CTNNB1
  • Gene Alias:
  • CTNNB,DKFZp686D02253,FLJ25606,FLJ37923
  • Gene Description:
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • Gene Summary:
  • Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000165222,OTTHUMP00000165223,catenin (cadherin-associated protein), beta 1 (88kD),catenin beta-1
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