CTNNB1 monoclonal antibody, clone M118
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More Files
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Western Blot (Cell lysate)
Western blot analysis of CTNNB1 immunoprecipitated from A-431 cells treated with pervanadate. The blots were probed with CTNNB1 monoclonal antibody, clone M118 (Cat # MAB1369). The immunoprecipitated CTNNB1 was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin fixed, citric acid treated parafin sections of embryonic rat E16 intestines. Sections were probed with CTNNB1 monoclonal antibody, clone M118 (Cat # MAB1369) then anti-mouse HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CTNNB1.
Immunogen
Recombinant protein corresponding to C-terminus of human CTNNB1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This peptide sequence is highly conserved in rat and mouse beta-Catenin.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:250)
Immunoprecipitation (5-10 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CTNNB1 immunoprecipitated from A-431 cells treated with pervanadate. The blots were probed with CTNNB1 monoclonal antibody, clone M118 (Cat # MAB1369). The immunoprecipitated CTNNB1 was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin fixed, citric acid treated parafin sections of embryonic rat E16 intestines. Sections were probed with CTNNB1 monoclonal antibody, clone M118 (Cat # MAB1369) then anti-mouse HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CTNNB1
Entrez GeneID
1499Protein Accession#
P35222Gene Name
CTNNB1
Gene Alias
CTNNB, DKFZp686D02253, FLJ25606, FLJ37923
Gene Description
catenin (cadherin-associated protein), beta 1, 88kDa
Gene Ontology
HyperlinkGene Summary
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM
Other Designations
OTTHUMP00000165222|OTTHUMP00000165223|catenin (cadherin-associated protein), beta 1 (88kD)|catenin beta-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Essential role of BCL9-2 in the switch between beta-catenin's adhesive and transcriptional functions.
Brembeck FH, Schwarz-Romond T, Bakkers J, Wilhelm S, Hammerschmidt M, Birchmeier W.
Genes & Development 2004 Sep; 18(18):2225.
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p120 Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin Interaction.
Piedra J, Miravet S, Castano J, Palmer HG, Heisterkamp N, Garcia de Herreros A, Dunach M.
Molecular and Cellular Biology 2003 Apr; 23(7):2287.
Application：IP, WB, Human, RWP1 cells.
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Regulation of E-cadherin/Catenin association by tyrosine phosphorylation.
Roura S, Miravet S, Piedra J, García de Herreros A, Duñach M.
The Journal of Biological Chemistry 1999 Dec; 274(51):36734.
Application：WB-Tr, Human, Caco-2 cells.
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Essential role of BCL9-2 in the switch between beta-catenin's adhesive and transcriptional functions.
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