Western blot analysis of CTNNB1 immunoprecipitated from A-431 cells treated with pervanadate. The blots were probed with CTNNB1 monoclonal antibody, clone M118 (Cat # MAB1369). The immunoprecipitated CTNNB1 was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies.
Formalin fixed, citric acid treated parafin sections of embryonic rat E16 intestines. Sections were probed with CTNNB1 monoclonal antibody, clone M118 (Cat # MAB1369) then anti-mouse HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM