AKT1 monoclonal antibody, clone M101
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-431 cells, serum starved overnight (lane 1) or treated with calyculin A (100 nM) for 30 min. (lane 2). The blot was probed with AKT1 monoclonal antibody, clone M101 (Cat # MAB1364).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant AKT1.
Immunogen
Recombinant protein corresponding to N-terminus of human AKT1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:250)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cells, serum starved overnight (lane 1) or treated with calyculin A (100 nM) for 30 min. (lane 2). The blot was probed with AKT1 monoclonal antibody, clone M101 (Cat # MAB1364).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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TNF induction of atrogin-1/MAFbx mRNA depends on Foxo4 expression but not AKT-Foxo1/3 signaling.
Moylan JS, Smith JD, Chambers MA, McLoughlin TJ, Reid MB.
American Journal of Physiology. Cell Physiology 2008 Oct; 295(4):C986.
Application：WB, Mouse , C2C12 cells.
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IFN-gamma does not mimic the catabolic effects of TNF-alpha.
Smith MA, Moylan JS, Smith JD, Li W, Reid MB.
American Journal of Physiology. Cell Physiology 2007 Dec; 293(6):C1947.
Application：WB, Mouse, C2C12 cells.
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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.
Powell DJ, Hajduch E, Kular G, Hundal HS.
Molecular and Cellular Biology 2003 Nov; 23(21):7794.
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TNF induction of atrogin-1/MAFbx mRNA depends on Foxo4 expression but not AKT-Foxo1/3 signaling.
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